Fig. 1.

WASH localises to early and late endosomal vesicles of A2780 ovarian cancer cells. (A) Cells were transfected with the indicated GFP-fused endosomal marker (green in ‘merge’ image) for 24 hours, fixed and stained for endogenous WASH (red in ‘merge’ image). Scale bars: 10 μm. (B) Relative colocalisation of WASH and the indicated endosomal markers was determined using an ImageJ plugin (see Materials and Methods). Values are the means of three independent experiments with n=15 each. Error bars indicate s.e.m.