Fig. 2.
Effect of M-cadherin RNAi on serum-starvation-induced apoptosis. M-cadherin RNAi (M-), non-targeted scrambled siRNA-transfected (SiCON) or normal control (NC) C2C12 myoblasts were serum starved from zero to 48 hours before being harvested. *P<0.05 vs both SiCON and NC control groups. The data are shown as the mean ± s.e.m. of three independent experiments. (A) Mitochondria were isolated from attached cells after serum starvation for 0 hours (SS-0h), 6 hours (SS-6h), 12 hours (SS-12h), 24 hours (SS-24h) or 48 hours (SS-48h). The mitochondria Δψm (orange to green ratio) was evaluated by FACS analysis of JC-1 staining. (B) DNA fragmentation in attached cells was measured by a cell death ELISA at the same time points as described in A. (C) The percentage of cells undergoing apoptosis in both attached and floating cells was defined as: (total TUNEL-positive attached cells + total TUNEL-positive floating cells)/(total attached cells + total floating cells).