Fig. 8.

Effect of M-cadherin RNAi and GSK-3β inhibition on apoptosis and myogenic differentiation of primary syndecan-4-positive myoblasts. Primary myoblasts were isolated from hindlimb muscles of 1-week-old C57BL/6 mice and syndecan-4-positive myoblasts were purified from isolated cells by fluorescence activated cell sorting (FACS). (A) Approximately 20.2% cells of adult stem cells that were isolated from hindlimb skeletal muscles were syndecan-4 positive (left). The post-sort verification of FACS sorted cells indicated that the purity of syndecan-4-positive cells was 97% (right). (B) Syndecan-4-positive myoblasts were grown on coverslips and treated as described in Fig. 7. The imaging of the cells was the same as in Fig. 7A. (C,D) Data from three independent experiments were given as mean ± s.e.m. *P<0.05 vs SiCON/DMSO. †P<0.05 vs M-/DMSO. (C) The myoblast fusion index was calculated as described in Fig. 7B. (D) The apoptotic index was calculated as described in Fig. 7C.