Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 15;124(22):3871–3883. doi: 10.1242/jcs.088625

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011.

PMC Copyright notice

Fig. 6. — Scheme of cell cycle-dependent CENP-N levels and its dynamic binding to kinetochores. (A) CENP-N is present at kinetochores at low levels (about 20%) during mitosis and G1. CENP-N exchanges fast at kinetochores during G1 and early S (yellow) and binds there stably during middle and late S phase with very slow loading (blue), probably due to CENP-L binding to the CENP-N C-terminus. CENP-N continuously dislocates from kinetochores during G2. (B) CENP-N binds to CENP-A-containing nucleosomes by fast exchange during early S phase (top left). In middle and late S phase (top right), CENP-N is stably bound due to an interaction of its C-terminus with a CCAN protein, probably CENP-L. Below: The C-terminal deletion mutant CENP-NΔC cannot be bound and fixed by CCAN (CENP-L). Accordingly, CENP-NΔC shows fast exchange also in middle S phase, with the same time constant as wild-type CENP-N in early S phase, and high recovery. A quantitative computer model is consistent with this interpretation (Bashar Ibrahim, Mathematics and Computer Science, FSU, Ernst-Abbe-Platz 1-3, 07743 Jena, Germany, personal communication; various assumptions of this model have to be verified experimentally).