Figure 5.

LncRNAs deregulated in pancreatic cancer metastasis map to intronic regions of genes associated with the MAPK pathway and/or related to apoptosis. Transcriptional orientation of eleven intronic lncRNAs mapping to MAPK pathway and/or apoptosis-related gene loci (ARRB1, ATF2, MAPK1, MAP2K5, MAP3K1, MAP3K14, PPP3CB, RAPGF2, TGFβR2, DAPK1 and PTEN) was investigated by strand-oriented reverse transcription followed by PCR. For each gene, sense (S) or antisense (AS) transcription was measured. Controls (C) for the absence of self-annealing during reverse transcription (RT) were obtained by performing RT reactions in the absence of primers (RT+, Primer -). Controls for the absence of genomic DNA contamination were obtained by omitting reverse transcriptase in the RT reaction (RT-, Primer +).