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. 2011 Nov 13;10:141. doi: 10.1186/1476-4598-10-141

Figure 6.

Figure 6

Expression changes of intronic lncRNAs mapping to PPP3CB, MAP3K14 and DAPK1 loci are not accompanied by changes in the corresponding protein-coding mRNAs. Relative levels of intronic lncRNAs and protein-coding mRNAs from PPP3CB (A), MAP3K14 (B) and DAPK1 (C) loci were determined by Real-Time PCR in clinical samples of primary adenocarcinoma (circles) or distant metastasis (squares) from pancreatic cancer patients. For each transcript, the number of tested samples is indicated in the × axis. For each gene, results are expressed as fold-change relative to the average expression of primary tumor samples. For each sample, qPCR assays were performed in triplicate and mean values are shown. House-keeping gene HMBS was used as the endogenous control for normalization across patient samples. All intronic transcripts (left panels) were differentially expressed in metastatic samples at a significance threshold of p< 0.05 (PPP3CB, p = 0.0259; MAP3K14, p = 0.0069; DAPK1, p = 0.0035). Protein-coding transcripts (right panels) were not significantly differentially expressed.