Table 2.
Proteins differentially expressed in hearts of ACF rats.
| Proteins downregulated in ACF | |||||
|---|---|---|---|---|---|
| Peptides (95% confidence) | Seq. Cov. | Accession | Protein name | Protein Fold-change (iTRAQ ratio) | mRNA fold-change |
| 53 | 54 | gi|259435950 | Long-chain-fatty-acid-CoA ligase 1 | 0.23 | NA |
| 17 | 25 | gi|59797483 | Carnitine O-acetyltransferase | 0.24 | 0.52 |
| 124 | 66.1 | gi|189083744 | Sarcomeric mitochondrial creatine kinase | 0.24 | 1.05 |
| 42 | 63 | gi|54035288 | Enolase 3, beta | 0.26 | 0.23 |
| 26 | 52 | gi|57333 | 3-2 trans-enoyl-CoA isomerase | 0.27 | 0.54 |
| 40 | 49 | gi|60688124 | Trifunctional enzyme subunit alpha, mitochondrial (HADHA) | 0.3 | 0.54 |
| 24 | 35 | gi|31077132 | Histidine rich calcium binding protein | 0.31 | 0.61 |
| 9 | 37 | gi|1906812 | Inducible carbonyl reductase | 0.32 | 0.45 |
| 49 | 65 | gi|56541110 | Acyl-Coenzyme A dehydrogenase, very long chain | 0.33 | 0.59 |
| 28 | 45 | gi|510110 | Trifunctional enzyme subunit beta, mitochondrial (HADHB) | 0.33 | 0.56 |
| 4 | 17 | gi|66910891 | Glutamic-pyruvate transaminase (alanine aminotransferase) | 0.34 | 0.38 |
| 113 | 53 | gi|57303 | Sarcoplasmic reticulum 2+-Ca-ATPase (SERCA2) | 0.35 | 1.0 |
| 40 | 56.8 | gi|149042663 | Sarcalumenin | 0.36 | 0.91 |
| 20 | 41.1 | gi|77993368 | Acyl-CoA synthetase family member 2 precursor | 0.39 | NA |
| 120 | 74.3 | gi|6978661 | Muscle creatine kinase | 0.4 | 0.69 |
| 195 | 75.8 | gi|83300587 | ATP synthase subunit alpha, mitochondrial; | 0.4 | 0.71 |
| 120 | 71 | gi|62079055 | Isocitrate dehydrogenase 2 (NADP+) | 0.41 | 0.62 |
| 30 | 50 | gi|7387725 | Medium and short chain L-3-hydroxyacyl-coenzyme A dehydrogenase | 0.43 | 0.37 |
| 18 | 47.5 | gi|51260066 | Propionyl coenzyme A carboxylase, beta polypeptide | 0.43 | 0.84 |
| 19 | 39 | gi|6166586 | Acyl-coenzyme A thioesterase 2 | 0.44 | 0.54 |
| 24 | 42.6 | gi|149050263 | Propionyl-CoA carboxylase alpha chain | 0.44 | 0.91 |
| 35 | 40.7 | gi|6978543 | Na+/K+ -ATPase alpha 1 subunit precursor | 0.45 | 1.1 |
| 34 | 64 | gi|56929 | Pyruvate kinase M1/M2 | 0.46 | 0.6 |
| 16 | 37 | gi|62825891 | Phosphofructokinase, muscle | 0.46 | 0.5 |
| 42 | 68.8 | gi|57527204 | Electron-transfer-flavoprotein, alpha polypeptide | 0.46 | 0.69 |
| 10 | 30 | gi|149062241 | LRP16 protein | 0.47 | 0.38 |
| 35 | 47.9 | gi|92090591 | Glutamate dehydrogenase 1 | 0.47 | 0.84 |
| 13 | 43 | gi|6981396 | Protein kinase, cAMP dependent regulatory, type I, alpha | 0.47 | 1.0 |
| 68 | 37 | gi|61557127 | Nicotinamide nucleotide transhydrogenase | 0.48 | 0.67 |
| 111 | 69.1 | gi|6978431 | Long-chain acyl-CoA dehydrogenase precursor | 0.49 | 0.84 |
| 31 | 49 | gi|48734846 | Acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain | 0.53 | 0.58 |
| 64 | 44.5 | gi|81883712 | 2-oxoglutarate dehydrogenase E1 component | 0.53 | 0.69 |
| 48 | 67 | gi|149027156 | Acetyl-Coenzyme A acyltransferase 2 | 0.54 | 0.61 |
| 45 | 25.9 | gi|189181710 | Ryanodine receptor 2, cardiac | 0.58 | 0.79 |
| 30 | 37 | gi|81871846 | Leucine-rich PPR motif-containing protein, mitochondrial | 0.61 | 0.66 |
| 33 | 45 | gi|6978705 | Carnitine O-palmitoyltransferase precursor | 0.61 | 0.58 |
| Proteins upregulated in ACF | |||||
| Peptides (95% confidence) | Seq. Cov. | Accession | Protein name | Protein Fold-change (iTRAQ ratio) | mRNA fold-change |
| 44 | 55 | gi|48425083 | Monoamine Oxidase A | 4.06 | 1.93 |
| 10 | 18 | gi|55249666 | Cadherin 13 | 3.40 | 2.15 |
| 19 | 34 | gi|5326787 | Transglutaminase 2 | 3.07 | 1.93 |
| 24 | 61 | gi|94400790 | Heat shock protein 1 (HSP27) | 3.05 | 1.41 |
| 23 | 72.2 | gi|438878 | tropomyosin | 3.04 | 1.32 |
| 10 | 42 | gi|6978501 | Annexin A1 | 3.00 | 2.23 |
| 35 | 69.6 | gi|535069 | Muscle LIM protein [Rattus norvegicus] | 2.97 | 1.31 |
| 22 | 50 | gi|6981324 | Prolyl 4-hydroxylase, beta polypeptide | 2.91 | 1.27 |
| 59 | 73.3 | gi|56388799 | Brain creatine kinase (Ckb protein) | 2.88 | 1.31 |
| 16 | 30 | gi|149048530 | Ceruloplasmin, isoform CRA_a | 2.80 | 2.02 |
| 34 | 62.3 | gi|744592 | Alpha-B crystallin | 2.61 | 1.05 |
| 35 | 68 | gi|157830232 | Annexin V | 2.58 | 1.71 |
| 20 | 27.3 | gi|462569 | Microtubule-associated protein 1A | 2.58 | 1.30 |
| 10 | 26.9 | gi|158706096 | Pre-B-cell leukemia transcription factor-interacting protein 1 | 2.45 | 1.23 |
| 8 | 34.4 | gi|68837285 | D-beta-hydroxybutyrate dehydrogenase, mitochondrial; | 2.44 | 1.02 |
| 10 | 28 | gi|974168 | Aldehyde dehydrogenase 1A1 (retinal dehydrogenase 1) | 2.43 | 1.84 |
| 11 | 28 | gi|7533042 | Guanine deaminase | 2.41 | 2.02 |
| 8 | 28.9 | gi|57241 | Sulfated glycoprotein 2 (clusterin) | 2.39 | 1.34 |
| 24 | 38.6 | gi|6981022 | Hexokinase 1 | 2.23 | NA |
| 59 | 64.4 | gi|109468300 | Alpha-enolase (Enolase 1) | 2.23 | 1.00 |
| 94 | 50.7 | gi|149063941 | Beta myosin heavy chain myo7 | 2.22 | 1.02 |
| 14 | 35.3 | gi|53237076 | EH-domain containing 4 | 2.22 | 1.08 |
| 22 | 50 | gi|9845234 | Annexin A2 | 2.21 | 2.17 |
| 11 | 25 | gi|149018456 | Microtubule-associated protein 4 | 2.18 | 1.24 |
| 6 | 26 | gi|158186676 | Calumenin isoform a | 2.17 | 0.86 |
| 39 | 42.6 | gi|54673763 | Heat shock protein 90, alpha (cytosolic), class A member 1 | 2.14 | 1.27 |
| 13 | 60.8 | gi|1051270 | 14-3-3 zeta isoform | 1.99 | 1.18 |
| 11 | 33.2 | gi|55855 | Calreticulin | 1.90 | 1.18 |
| 41 | 26.9 | gi|62646949 | Filamin-C (Gamma-filamin) (Filamin-2) | 1.87 | 1.21 |
| 18 | 39.1 | gi|157819677 | Sarcolemma associated protein | 1.81 | 1.02 |
Identification of all proteins was based on at least four peptides. (for peptide sequences see Additional data 2). NA- mRNA not represented on the chip.