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Characterization of the optimized biosensor. (A-B) Emission scans of H3K27-MetBio2 (A) and H3K27-MetBio3 (B) after treatment with vSET in the presence or absence of SAM, normalized to acceptor intensity. (C) Time course of FRET ratio changes for H3K27-MetBio variants treated with vSET and SAM and calculated using the ratio of fluorescence intensities at 526 nm and 494 nm.
