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Purified strep-tagged MeCP2 (rat) and purified intein tagged MECP2 (human) were subjected to a SDS-PAGE and stained with Coomassie. The molecular weight markers are labeled in the middle. A schematic representation of the rat MeCP2 protein and its functional domains is shown below. MBD: methyl CpG binding domain; TRD: transcriptional repression domain; NLS: nuclear localization signal.
