Figure 4. Antibody specificity.

A) Sequence alignment of MeCP2 from different species. Identical residues are shaded in gray. The identities range from 93% (human-mouse) to 97% (rat-mouse). B) For a multi-species immunoblot nuclear extracts from pig, mouse and rat brain (10⁶ nuclei) were loaded and probed with the antibodies as indicated. C) For immunoprecipitation analysis, mouse brain whole cell (for rat antibodies) and nuclei (for mouse antibodies) extracts were incubated with the monoclonal antibodies as indicated followed by western blot analysis.