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. 2011 Nov 28;6(11):e27916. doi: 10.1371/journal.pone.0027916

Figure 5. Identification of tasiARF-containing TAS3 olive genes and putative ARF target sequences.

Figure 5

(A) Schematic alignment of predicted transcripts and ESTs for TAS3 genes (tasiARF precursors) from olive and other seed plants. At least two olive cDNA clones (contig #48241 was shown as a representative) contain dual miR390 complementary sites that flank the area of predicted tasiRNA production. The regions corresponding to the 5′ miR390 complementary site, tasiARFs, and the 3′ miR390 complementary site are expanded. tasiARF refers to the regions homologous to Arabidopsis TAS3 5′D7(+) and TAS3 5′D8(+). miR390 binding sites are indicated. (B) Alignment of ARF-like target genes from olive in the regions of sequence complementarity to tasiARF. Three putative ARF contigs in the olive database are shown to contain each two separated tasiARF complementary sites. Alignments and color-coded based on the confidence of the local alignment were generated using T-Coffee and its CORE function.