Table 3. Peak expression and time point of candidate genes associated with IFN response that were modulated in IFNα2 pre-treated macrophages compared to untreated control and modulated by HIV-1.
| IFN-relatedgenes | ReferenceSequence | Peak expressionIFNα2/CTRL(Fold induction) | Time(hours) | Peak expressionIFNα2/HIV infection(Fold induction) | Time(hours) |
| BIRC3 | NM_001165 | 7 | 2 | 3,5 | 2 |
| CCL5 | NM_002985 | 8 | 2 | 4 | 8 |
| CD38 | NM_001775 | 39 | 4 | 13 | 16 |
| CEBPD | NM_005195 | 2 | 8 | 1 | 16 |
| CHI3L1 | NM_001276 | 2 | 2 | 1 | 2 |
| CXCL10 | NM_001565 | 124 | 2 | 31 | 2 |
| CXCL11 | NM_005409 | 118 | 2 | 39 | 8 |
| CXCL9 | NM_002416 | 28 | 2 | 9 | 16 |
| EHF | NM_012153 | 4 | 8 | 1 | 16 |
| HMGA1 | NM_145899 | 2 | 8 | 1 | 24 |
| LILRB2 | NM_005874 | 16 | 8 | 8 | 2 |
| NCOA3 | NM_181659 | 2 | 2 | 4 | 4 |
| OGT | NM_181632 | 5 | 8 | 4 | 2 |
| PIGR | NM_002644 | 3 | 8 | 1,5 | 8 |
| PIP5K2A | NM_005028 | 3 | 8 | 1,5 | 8 |
| SAMHD1 | NM_015474 | 2 | 8 | 1 | 8 |
| TAP2 | NM_000544 | 16 | 2 | 8 | 2 |
| TCF4 | NM_003199 | 2 | 2 | 0,7 | 16 |
| TRAF6 | NM_004620 | 2 | 8 | 1 | 8 |
| TXNIP | NM_006472 | 2 | 8 | 1 | 16 |
| VISA | NM_020746 | 2 | 8 | 1 | 8 |
Selection of the top 500 most modulated genes when comparing the IFNα2 pre-treated macrophages infected or not with HIV-1. Of these, 55 were related to interferon as a keyword. From these 55, 21 were upregulated by interferon as compared to untreated control (peak expression and time point) but modulated differentially when HIV-1 was added. Those genes may represent interferon effectors that are modulated by HIV-1 infection during the interferon response.