Figure 4. SAHA treatment decreases HDAC2 protein levels but does not affect the protein levels of other class I and IIa HDACs – 2nd trial.
HDAC2 protein levels are reduced in the cortex (A) and brain stem (B) but not in the hippocampus (data not shown) of WT and R6/2 SAHA as compared to vehicle treated mice. Representative western immunoblots of 20 µg of cortical and brain stem homogenates from 9-week-old WT and R6/2 SAHA and vehicle treated mice are shown. HDAC1, HDAC3, HDAC5, HDAC7 and HDAC9 protein levels do not change upon SAHA treatment of WT or R6/2 mice in the cortex (A) nor in the brain stem or hippocampus (data not shown). In all cases, HDAC protein levels were quantified using densitometry and normalized to alpha-tubulin. Error bars represent S.E.M. (n = 4).