Figure 6. CD133 downregulation and Src inhibition abrogate the p-Src activity and sphere formation ability in primary HN-CICs.

(A) Protein level of CD133 and p-Src of lentivius mediated CD133 knockdown primary HN-CICs was analyzed by western blot. (B) Primary HN-CICs were first infected with sh-Luc or CD133-shRNA lentivirus. Three days after the lentiviral infection, the sphere formation ability of virus infected cells then cultivated under selection medium were recorded. (C) Newly enriched primary HN-CICs were treated with PP2 (10 µM) for 72 hrs and the sphere formation ability of PP2 treated HN-CICs cells were examined. Arrows indicated the sphere cells.