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. 2011 Nov 28;6(11):e27910. doi: 10.1371/journal.pone.0027910

Figure 4. eNOS activity.

Figure 4

The left panel shows representative Western blots performed using antibodies against total eNOS protein and phosphorylated eNOS, with β-actin as loading control. All blots were subjected to densitometry and the right panel shows the average expression of total eNOS (top) and phosphorylated eNOS (bottom). The P value in the insets reports ANOVA. *, P<0.05 when comparing untreated chronic hypoxia vs. normoxia; #, P<0.05 when comparing the various treatments vs. untreated chronic hypoxia. All post-tests performed with the Bonferroni method.