Figure 4.

CD8+ T-cell response obtained by ICS in rAd5-CE1E2 and rTTV-CE1E2 immunised mice. Splenocytes isolated at week 20 were stimulated in vitro with peptide E1-21 (SQLFTFSPRRYETI) alone. Representative fluorescence-activated cell sorter flowgrams are shown in Figure 4A (percentages of IFN-γ+CD8+cells are shown in the right upper quadrants), and a summary of the percentages of IFN-γ-producing CD8+cells are presented in Figure 4B as the mean with SEM of four mice per group. Significant p values between the vaccinated groups are shown. * indicates p < 0.05, ** indicates p < 0.01.