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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Fertil Steril. 2011 Jul 13;96(3):715–721. doi: 10.1016/j.fertnstert.2011.06.021

Figure 2. Reactivity of 30 C. trachomatis antigens with 54 patient sera at 1:4000 dilution.

Figure 2

The 30 antigens were reacted with the 54 human sera as described in table Table 2 note except that each serum was diluted 1:4000 (data not shown). (A) Ten of the 30 antigens, failing to react with any of the 23 IFC sera, were thus presented in the figure. Note that HSP60 (CT110) and OmcB (CT443) maintained a detection sensitivity of 35.5% and 58% respectively. (B) The reactivity of each of the 10 antigens was analyzed at individual antiserum level. Note that the combinations of CT443 with CT381 or HSP60 with CT376, CT381 & CT798 can have the highest sensitivity of 67.7% while maintaining 100% specificity. (C) The reactivity intensity between each antigen and the 21 positive sera (measured at individual antiserum level) was expressed as mean OD plus standard deviation. (D) Each of the 10 antigen were reacted with an antiserum sample pooled from the 21 sera at equal ratio without (D) or with absorption with C. trachomatis (CT)-infected HeLa lysate (E) or HeLa alone lysate (F). Note that absorption with CT-HeLa but not HeLa alone lysates removed the reactivity of each antigen with the pooled antiserum.