Table 2.

Reactivity of 30 significant C. trachomatis proteins with 31 TFI & 23 IFC patient antisera (@1:800)

The 30 C. trachomatis antigens significantly recognized by 24 TFI patients as identified in Fig.1 were reacted with 54 patient sera (33 TFI and 21 IFC). All sera were diluted at 1:800 regardless of their over anti-C. trachomatis antibody titers as determined with the immunofluorescence assay. The mean ODs of each antigen were compared between TFI and IFC groups were compared using Student t-Test and the corresponding p values were listed in the last column of the left panel, confirming that all 30 antigens were significantly recognized by TFI patients. The number of positive recognition (determined as described Fig. 1 legend) by either TFI or IFC was used to calculate recognition specificity and sensitivity as well as positive or negative predicting values (PPV or NPV; right panel). Note that HSP60 (CT110) displayed a detection specificity of 82.6% and sensitivity of 71% and many other immundominant antigens such as pCT03 (Pgp3, a plasmid-encoded secreted protein), CT858 (CPAF, a chlamydial protease/proteasome-like activity factor that is secreted into host cell cytosol), CT823 (cHtrA, a secreted stress response serine protease), CT813 (an inclusion membrane protein), CT443 (OmcB, outer membrane complex protein B) & CT143 (a hypothetical protein) behaved similarly. Only the hypothetical protein CT557 had a 100% specificity but its sensitivity was only 29%. Thus, under this assay condition, no single antigen or combinations of antigens can achieve 100% specificity with a sensitivity of >50%.

CT	TFI (n=31)	IFC (n=23)	t-Test	Specificity	Sensitivity	PPV	NPV
ORF	X +/− SD	X +/− SD
CT067	0.407 ± 0.483	0.045 ± 0.115	<0.001	87.0%	51.6%	84.2%	57.1%
CT089	0.645 ± 0.936	0.189 ± 0.407	0.020	78.3%	45.2%	73.7%	51.4%
CT110	0.679 ± 0.756	0.069 ± 0.112	<0.001	82.6%	71.0%	84.6%	67.9%
CT116	0.176 ± 0.252	0.012 ± 0.059	0.001	95.7%	32.3%	90.9%	51.2%
CT119	0.375 ± 0.501	0.082 ± 0.196	0.005	87.0%	48.4%	83.3%	55.6%
CT142	0.468 ± 0.522	0.098 ± 0.138	0.001	78.3%	54.8%	77.3%	56.3%
CT143	1.012 ± 0.818	0.166 ± 0.220	<0.001	73.9%	71.0%	78.6%	65.4%
CT147	0.789 ± 0.678	0.303 ± 0.185	0.001	34.8%	80.6%	62.5%	57.1%
CT153	0.404 ± 0.561	0.071 ± 0.110	0.003	91.3%	45.2%	87.5%	55.3%
CT322	0.366 ± 0.586	0.055 ± 0.112	0.007	95.7%	41.9%	92.9%	55.0%
CT376	0.453 ± 0.616	0.072 ± 0.097	0.002	95.7%	41.9%	92.9%	55.0%
CT381	0.330 ± 0.346	0.059 ± 0.074	<0.001	95.7%	51.6%	94.1%	59.5%
CT414	0.327 ± 0.469	0.061 ± 0.082	0.004	95.7%	51.6%	94.1%	59.5%
CT442	0.486 ± 0.622	0.055 ± 0.070	0.001	91.3%	48.4%	88.2%	56.8%
CT443	1.145 ± 1.020	0.110 ± 0.173	<0.001	87.0%	71.0%	88.0%	69.0%
CT456	0.803 ± 0.879	0.241 ± 0.558	0.006	73.9%	64.5%	76.9%	60.7%
CT529	0.854 ± 0.644	0.444 ± 0.310	0.003	13.0%	87.1%	57.4%	42.9%
CT557	0.358 ± 0.638	0.028 ± 0.057	0.007	100%	29.0%	100%	51.1%
CT603	0.579 ± 0.654	0.141 ± 0.161	0.001	78.3%	61.3%	79.2%	60.0%
CT681	0.363 ± 0.386	0.060 ± 0.078	<0.001	87.0%	51.6%	84.2%	57.1%
CT694	0.698 ± 0.848	0.150 ± 0.293	0.002	78.3%	54.8%	77.3%	56.3%
CT795	0.647 ± 0.771	0.034 ± 0.101	<0.001	95.7%	61.3%	95.0%	64.7%
CT798	0.622 ± 0.827	0.038 ± 0.089	<0.001	91.3%	51.6%	88.9%	58.3%
CT806	0.673 ± 0.772	0.104 ± 0.212	<0.001	82.6%	54.8%	81.0%	57.6%
CT812	0.555 ± 0.667	0.061 ± 0.087	<0.001	91.3%	54.8%	89.5%	60.0%
CT813	0.673 ± 0.689	0.095 ± 0.165	<0.001	78.3%	67.7%	80.8%	64.3%
CT823	0.649 ± 0.709	0.071 ± 0.074	<0.001	91.3%	71.0%	91.7%	70.0%
CT858	1.947 ± 1.276	0.338 ± 0.666	<0.001	78.3%	74.2%	82.1%	69.2%
CT866	0.574 ± 0.738	0.062 ± 0.079	0.001	91.3%	48.4%	88.2%	56.8%
pCT03	1.761 ±1.366	0.166 ± 0.573	<0.001	82.6%	74.2%	85.2%	70.4%