Table II.

Pure MTX, Unloaded, and MTX-Loaded NPs Induced Cytotoxicity in C6 Cells

	IC50 (μg/ml) in C6 cell line
Unloaded NPs CS	414 (±49)*
Unloaded NPs CS Tween 80	766 (±37)*
Unloaded NPs GCS	141 (±1)*
Unloaded NPs GCS Tween 80	80 (±37)*
MTX-loaded NPs CS	0.11 (±0.02)*
MTX NPs-loaded CS Tween 80	0.08 (±0.009)*
MTX-loaded NPs GCS	0.04 (±0.02)*
MTX-loaded NPs GCS Tween 80	0.09 (±0.002)*
Pure MTX	0.0009 (±0.0005)

The cells were treated with pure MTX, unloaded NPs, and MTX-loaded NPs for 24 h. The cell viability was measured via the MTT assay and compared to a control (i.e., pure MTX). Values represent means±SD (n = 8)

*p < 0.001, significantly different from MTX control