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. Author manuscript; available in PMC: 2013 Jan 18.
Published in final edited form as: Physiol Behav. 2011 Aug 17;105(2):234–241. doi: 10.1016/j.physbeh.2011.08.007

Figure 1.

Figure 1

Adeno-associated virus (AAV)-mediated expression of cholecystokinin (CCK) 1 receptor (CCK1R). (A) Construct of AAV-mediated CCK1R expression vector (AAVCCK1R) containing the CCK1R gene driven by the CMV promoter and the expression marker of hrGFP translationally controlled by the IRES; (B) Both CCK1R and hrGFP produced in the AAVCCK1R-infected cells three days after viral infection as determined by Western blot; (C) Representative micrograph shows hrGFP expression in the dorsomedial hypothalamus (DMH) two weeks after DMH viral injection as examined under fluorescence microscopy; (D) Quantitative Cck1r mRNA expression in the DMH of Otsuka Long-Evans Tokushima Fatty (OLETF) receiving AAVCCK1R (OLETF-AAVCCK1R) 14 weeks post-viral injection relative to lean control Long-Evans Tokushima Otsuka (LETO) rats receiving control vector AAVGFP (LETO-AAVGFP) as determined by in situ hybridization determination. Values are means ± SEM. n= 6~8 animals in each group.