The anorexigenic effect of centrally infused mCPP is increased by estradiol treatment in OVX rats. Dark-phase food intake was measured at 1 and 22 h after i.c.v. infusion of aCSF and 250 μg mCPP in oil- and estradiol-treated OVX rats. (A) mCPP decreased 1-h dark-phase food intake in estradiol-treated, but not oil-treated, OVX rats. (B) mCPP decreased 22-h dark-phase food intake in oil- and estradiol-treated rats, however, the magnitude of this effect was increased by estradiol treatment. Data are means ± SEMs. *Less than oil/aCSF, P < 0.05. **Less than estradiol/aCSF, P < 0.01. #Less than oil-treated rats, P < 0.05.