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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Neurotoxicol Teratol. 2011 Aug 4;33(6):715–720. doi: 10.1016/j.ntt.2011.07.010

Figure 3. Altered transcript level of axonogenesis genes following Pb treatment at different developmental time points.

Figure 3

Transcript levels for five genes of interest were measured at seven developmental time points with qPCR. Data were quantified with standard curves using gapdh as a reference gene and normalized to control treatment. Transcript levels of shha and epha4b were significantly altered at 14 hpf (A) and 16 hpf (B), respectively. Netrin2 was significantly altered at 30 and 36 hpf (C and D). No differences were detected in gene expression at 18, 20 or 24 hpf for shha, epha4b or netrin2 (data not shown) or in netrin1b or noi at any time point analyzed (data not shown). n=6 (50 pooled embryos for each replicate per time point per group). *p<0.05, Pb treatment compared to control