Fig. 8.

(A) NeuN Expression in Dorsal Cortex from Tsc1^Emx1-Cre CKO and control mice. Total dorsal cortical extracts express similar levels of NeuN in P15 control and Tsc1^Emx1-Cre CKO mice. Immunoblots using extracts from three control and Tsc1^Emx1-Cre CKO mice are shown. Actin is used as a loading control. (B) Rapamycin decreases neuronal area in NeuN positive cells from control and Tsc1^Emx1-Cre CKO brains treated with rapamycin. Rapamycin withdrawal allows further increases in area in CKO mice. Animals were treated with rapamycin form P15-40 (“on rapa”). An additional group of mice were treated from P15-40 and then euthanized at P55 approximately two weeks after cessation of rapamycin (“off rapa”). Asterisk denotes statistical significance using Students t-test, p<0.05.