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Our exposure regimen for 0-5 dpf exposure using CPF, DZN and PA along with normal development of zebrafish. Compared to Yang et al. (2011) whose exposures start at 24 hpf, our exposures begin at 6 hpf. At 6 hpf, gastrulation has just begun and neurons are not formed. At 24 hpf, somitogenesis is ending and a nervous system is rapidly developing. After a 0-5 day exposure, larvae are washed and tested for motility at 6 dpf.
