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. Author manuscript; available in PMC: 2013 Jan 18.
Published in final edited form as: Physiol Behav. 2011 Sep 10;105(2):417–427. doi: 10.1016/j.physbeh.2011.08.041

Table 1. Treatment groups and numbers of animals.

Group Sub-Group N through EXT Conditioning Extinction (EXT) N at SR SR Test Solution
Days 1,3, 5 Days 2, 4, 6 Odd Days Even Days
Acute DCS CSO (2–5%) 11 SAC1+ LiCl2 Water SAC+ SAL/DCS3 Water+ (SAL+SAL/DCS)4 6 SAC
CSO (8- 16%) 10 SAC+ LiCl Water SAC+ SAL/DCS Water+ (SAL+SAL/DCS) 5 SAC
CSO (20–40%) 9 SAC+ LiCl Water SAC+ SAL/DCS Water+ (SAL+SAL/DCS) 7 SAC
EU (2–5%) 12 SAC+ LiCl Water SAC+ SAL/DCS Water+ (LiCl+SAL/DCS)5 7 SAC
EU (8–16%) 13 SAC+ LiCl Water SAC+ SAL/DCS Water+ (LiCl+SAL/DCS) 9 SAC
EU (20–40%) 15 SAC+ LiCl Water SAC+ SAL/DCS Water+ (LiCl+SAL/DCS) 13 SAC
Chronic DCS CSO 11 SAC+ LiCl Water SAC+ DCS Water+ (SAL+DCS)6 6 SAC
EU 10 SAC+ LiCL Water SAC+DCS Water + (LiCl+DCS)7 6 SAC
Saline Control CSO 10 SAC + LiCl Water SAC+SAL Water + (SAL+SAL)8 6 SAC
EU 9 SAC+ LiCl Water SAC+SAL Water+ (SAL+LiCl)9 6 SAC
1

SAC= saccharin dissolved in deionized water (0.3%), presented in 30 minute exposures.

2

LiCl= intraperitoneal injection, 81 mg/kg at a volume of 1 ml/kg, lithium chloride dissolved in physiological saline.

3

SAL/DCS = .9% physiological saline intraperitoneal injection (NaCl dissolved in deionized water) give at 1 ml/kg or DCS intraperitoneal injection (15 mg/kg given at 1 ml/kg, dissolved in physiological saline). Rats are given SAL control injections everyday excluding the four days they received their DCS treatment injections.

4

(SAL+SAL/DCS) = Two injections given within 30 seconds of each other, one SAL i.p. 1ml/kg and then one i.p. injection of either SAL 1 mg/kg or DCS 15 mg/kg at 1 ml/kg, depending on whether in DCS treatment or not.

5

(LiCl+SAL/DCS) = Two injections given within 30 seconds of each other, one LiCl i.p 81mg/kg at a volume of 1 ml/kg and one i.p injection of either SAL 1 mg/kg or DCS 15 mg/kg at 1 ml/kg, depending on whether in DCS treatment or not.

6

(SAL+DCS) = Two injections given within 30 seconds of each other, one SAL i.p. 1ml/kg and then one i.p. injection of DCS 15 mg/kg at 1 ml/kg.

7

(LiCL+DCS) = Two injections given within 30 seconds of each other, one LiCl i.p 81mg/kg at a volume of 1 ml/kg and one i.p injection of DCS 15 mg/kg at 1 ml/kg.

8

(SAL+SAL) = Two injections SAL i.p. 1 ml/kg given within 30 seconds of each other

9

(SAL+LiCl) = Two injections given within 30 seconds of each other, one SAL i.p. 1 ml/kg and then one LiCl i.p. 81 mg/kg at a volume of 1 ml/kg