Table 1. Quantitative distribution of functional groups in EB and RB proteomes.
| Functional groups | EB - fmol/μg, (Rank) | RB - fmol/μg, (Rank) |
|---|---|---|
| Translation | 4661 ± 45, (1) | 6440 ± 462, (1) |
| Virulence & T3S-related | 2903 ± 37, (2) | 1310 ± 115, (6) |
| Cell Envelope | 2463 ± 31, (3) | 3063 ± 225, (3) |
| Protein Folding, Assembly & Modification | 2362 ± 72, (4) | 3513 ± 85, (2) |
| Energy Metabolism | 2300 ± 21, (5) | 777 ± 42, (9) |
| Chlamydia-Specific Hypothetical Proteins | 1503 ± 28, (6) | 1425 ± 115, (5) |
| Miscellaneous Enzymes&Conserved Proteins | 1449 ± 37, (7) | 1042 ± 58, (7) |
| Transcription | 1256 ± 12, (8) | 929 ± 65, (8) |
| Base & Nucleotide Metabolism | 530 ± 10, (9) | 208 ± 42, (12) |
| Transport | 516 ± 11, (10) | 1494 ± 70, (4) |
| DNA Replication, Modification, Repair & Recombination | 478 ± 27, (11) | 436 ± 28, (10) |
| Central Intermediary Metabolism | 182 ± 7, (12) | 58 ± 2, (15) |
| Standard Protein Secretion | 181 ± 10, (13) | 428 ± 83, (11) |
| Amino Acid Biosynthesis | 152 ± 7, (14) | 120 ± 16, (13) |
| Signal Transduction | 112 ± 5, (15) | 32 ± 6, (16) |
| Biosynthesis of Cofactors | 83 ± 4, (16) | 118 ± 9, (14) |
| Cell Division | 54 ± 4, (17) | 18 ± 3, (17) |
All C. trachomatis L2 proteins quantified were classified into functional categories as described in Experimental procedures. The total mass for each category in EB and RB forms was calculated (fmol/μg) and expressed as the mean ± standard deviation resulting from 4 determinations (2 biological replicates processed in duplicate). Functional groups were then ranked based on abundance. Protein groups ranked within the top 6, representing 76.4% and 80.5% of the total mass in EB and RB, respectively, are highlighted in bold.