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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Exp Eye Res. 2011 Oct 18;93(6):880–888. doi: 10.1016/j.exer.2011.10.001

Figure 1. Recombinant Expression of COL8A2 Protein by Transfected CHO Cells.

Figure 1

Figure 1

CHO cells were transfected with wild-type and mutant COL8A2 plasmids [n=5]. [A], levels of monomeric 75kD FLAG-tagged collagen VIII, normalized to actin levels, were similar in all genotypes. Levels of trimeric collagen VIII [250kD], normalized to actin levels, were significantly higher in the mutant genotypes. There was no significant difference in the levels of NPTII between the COL8A2 genotypes, indicating consistent levels of transfection across genotypes. [B], the ratio of trimeric to monomeric collagen VIII was calculated and found to be significantly higher in the mutant genotypes. *p<0.02. Error bars, S.E.M.