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Hypoxia results in lower NO production in hPMVEC. hPMVEC in T75 flasks were incubated in either normoxia or hypoxia for 24 hours. The media was harvested for determination of nitrites using chemiluminescence, and the hPMVEC were harvested for protein determination. Nitrite production is presented in nmol per mg protein. * hypoxia different from normoxia, p<0.01.
