Figure 3.
Hypoxia decreased Vmax for L-arg uptake. A) L-arg uptake determined in hPMVEC incubated with varying amounts of L-arg during normoxia (black circles) and hypoxia (grey circles). L-arg uptake by hPMVEC is a saturable process which follows Michaelis-Menton kinetics, and hypoxia decreased L-arg uptake. B) Hypoxia had no effect on the Km of L-arg uptake by hPMVEC. C) Hypoxia decreased the Vmax of L-arg uptake by hPMVEC. * hypoxia different from normoxia, p<0.05.