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. Author manuscript; available in PMC: 2012 Dec 21.
Published in final edited form as: J Proteomics. 2011 Aug 16;75(2):410–424. doi: 10.1016/j.jprot.2011.08.007

Figure 10. Immunoblotting of nuclear protein enriched fraction.

Figure 10

Noise-induced increases in nuclear proteins were verified by immunoblotting a nuclear fraction of cochlear protein extract. A 1.7 fold increase was detected for a single WSTF-positive protein. E2F3 showed multiple bands possibly due to different E2F gene products while a 61 kDa protein, which is close to the predicted molecular weight changed by 1.3 fold. Noise also induced a 1.3 fold increase in Src protein. The images were cropped to show the particular bands as except for E2F3 all other proteins were specific. Actin was used to normalize the protein expression levels. The images are representative samples from two replicates.