Fig. 7.

Cellular metabolites affect nickel-dependent damage to FbaA. (A) As purified FbaA (20 nM) was challenged with 0 µM (open symbols) or 16 µM (closed symbols) Ni(II) in the absence (squares) or presence of 100 µM His (circles) or 1 mM reduced glutathione (GSH; triangles) aerobically at 25 °C, and the enzyme was assayed at the indicated times. The error bars represent the standard deviation of three independent experiments. (B) Mutant E. coli cells LEM201 (rcnA, squares) and LEM267 (gshA rcnA, circles) were grown aerobically at 37 °C in M9 glucose medium and challenged with 0 µM (open symbols) or 1 µM (closed symbols) Ni(II). The data are representative of three independent experiments.