TABLE 1.
E. coli strains and plasmids used
| Strains | Genotype | Source |
|---|---|---|
| BL21 (DE3) | dcm ompT hsdS(rB− mB−) gal λ(DE3) | Stratagene |
| Δ(araD-araB)567 ΔlacZ4787(::rrnB-3) rph-1 | ||
| BW25113 | Δ(rhaD-rhaB)568 hsdR514 | (Datsenko and Wanner, 2000) |
| JW2093-1 | BW25113 plus ΔrcnA731::kan | (Baba et al., 2006) |
| JW2011-2 | BW25113 plus Δgnd-727::kan | (Baba et al., 2006) |
| LEM201 | MG1655 plus ΔrcnA731::kan | This study |
| LEM202 | MG1655 plus Δgnd-727::kan | This study |
| LEM232 | MG1655 plus pLEM1 | This study |
| LEM233 | MG1655 plus ΔrcnA731::kan pWKS30 | This study |
| LEM234 | MG1655 plus ΔrcnA731::kan pLEM1 | This study |
| LEM267 | MG1655 plus ΔrcnA731::kan ΔgshA::cat | This study |
| LEM273 | MG1655 plus Δ(lacZ1)1 ΔrcnA731 pJI114 | This study |
| LEM276 | BL21 (DE3) plus pLEM5 | This study |
| LEM277 | BL21 (DE3) plus pLEM6 | This study |
| LEM278 | BL21 (DE3) plus pLEM7 | This study |
| LEM279 | BL21 (DE3) plus pLEM8 | This study |
| LEM280 | BL21 (DE3) plus pLEM9 | This study |
| MG1655 | F− λ− wild type | (Neidhardt, 1996) |
| SJ99 | As MG1655 plus Δ(lacZ1::cat)1 | (Anjem et al., 2009) |
| SSK112 | As BW25113 plus ΔgshA::cat | Jim Imlay |
| Plasmids | ||
| pET28b | PT7 His6-tag kmr | Novagen |
| pJI114 | pACYC163 plus rcnR-PrcnA-lacZ | (Iwig et al., 2006) |
| pLEM1 | pWKS30 plus fbaA inserted into SacI-EcoRI. | This study |
| pLEM5 | pET28b plus fbaA inserted into XhoI-BamHI. His6-tagged. | This study |
| pLEM6 | D144A mutant of pLEM5. | This study |
| pLEM7 | E174A mutant of pLEM5. | This study |
| pLEM8 | C177A mutant of pLEM5. | This study |
| pLEM9 | E181A mutant of pLEM5. | This study |
| pWKS30 | Plac polylinker Amr | (Wang and Kushner, 1991) |