Figure 1.

(A) Amino acid sequence of rat Kir6.2 showing the residues mutated in this study. (B) Response to application of ATP (1, 0.1 and 0.01 mM) of mutant channels assessed by inside-out patch voltage-clamp recording. Recording was performed in symmetrical Kint/EDTA solutions, with differing concentrations of ATP added to the bath solution. Error bars represent SEM, except R50E//E128K 1 mM ATP which is the difference between the individual values from the two patches tested; the number of patches tested (n) for each condition is given below the x-axis. WT: wild-type; EK: E128K.