Figure 5. dAdar and dfmr1 genetically interact.

(a) Confocal images of L3 larval NMJs from the following genotypes: WT, dAdar^5G1, dfmr1³, dfmr1(4X), dAdar^5G1;dfmr1³ and dAdar^5G1;dfmr1(4X). L3 larvae were stained for CSP (presynaptic, magenta) and DLG (postsynaptic, green). Type 1b and type 1s synaptic boutons were distinguished as described in Fig. 2. No noticeable differences in CSP and DLG intensity were observed across genotypes. Scale bar represents 50 μm. (b) Genetic studies demonstrating that dAdar is epistatic to dfmr1 with respect to synaptic bouton formation. Quantification of average number of type 1b (black bars) and type 1s (white bars) synaptic boutons for genotypes shown in (a). (c) Genetic studies demonstrating that dAdar is epistatic to dfmr1 with respect to primary branch length. Relative primary branch length was quantified for all genotypes shown in (a) using anti-HRP staining. The length of the primary branch length was normalized to the length of the abdominal hemisegment, and mean relative primary branch length was measured and plotted for each genotype. All images and quantification were performed using muscles 6/7, hemisegment A3. n≥16 for each genotype. Error bars denote s.e.m. *p<0.05, **p<0.01, ***p<0.001, analyzed by one-way ANOVA, p<0.0001 overall, Tukey-Kramer post-test.