Figure 1. Validation and metabolic phenotypes of NCoR1^skm-/- mice.

(A) mRNA levels of NCoR1 and Smrt in different tissues were determined by qRT-PCR. Values were normalized to 36B4. (n=8-10/group). (B) Biochemical analysis of the plasma from NCoR1^skm+/+ and ^skm-/- mice after 6 hr fasting (n=8) either on chow diet (CD) (top) or HFD (bottom). (C) Circadian activity, measured as the total locomotor activity, and energy expenditure was evaluated by the measurement of oxygen consumption (VO₂), and by the calculation of the respiratory exchange ratio (RER) over a 24 hr period after 12 wks of HFD. The bar graphs represent the average for each group (n = 12). (D) Body temperature was measured for 7 hr in mice exposed to 4°C after 18-wks of HFD (n=7, 8). (E and F) Exercise experiments, measuring running time and distance till exhaustion (endurance exercise) (E) and the increment of VO_2max, during exercise, and RER levels at basal and VO_2max condition (F) were performed after 14- and 17-wk HFD.

Data are expressed as mean ± SEM.

See also Suppl. Figs. 1-3 and Suppl. Tables 1-3.