Figure 6. Enhanced MEF2 activity in NCoR1^skm-/- muscle.

(A) Gene expression of myogenesis-related genes was measured by qRT-PCR in NCoR1^skm+/+ and ^skm-/- quadriceps (n=10). (B, C, and D) Acetylation levels of MEF2D were determined by Western blot after immunoprecipitation with an Ac-Lys Ab from gastrocnemius (B), from NCoR1^L2/L2-MEFs infected with Ad-GFP or Ad-Cre recombinase (C, right), and from C2C12 myotubes infected with Ad-shLacZ or Ad-shNCoR1 (D). MEF2D expression in total protein extracts was shown in the lower panels. The expression of NCoR1 and actin in NCoR1^L2/L2-MEFs was also shown (C, left). (E) MEF2 target mRNAs determined by qRT-PCR in C2C12 myotubes infected with either Ad-shLacZ or Ad-shNCoR1 (n=6). (F) NCoR1 recruitment to the MEF2 site of the mouse Mb promoter determined by ChIP in C2C12 myotubes. (G) Binding of either global acetylated histone 4 (H4) or H4 acetylated on K16 (H4K16) to the MEF2 site of the Mb gene was evaluated by ChIP from C2C12 myotubes infected as in (E). Data are expressed as mean ± SEM.

See also Suppl. Fig. 6 and Suppl. Table 3.