Figure 5.

Repeated ethanol administration did not alter the protein levels of NR1 or NR2A subunits in the DMS. (A and B) Sprague-Dawley rats were treated with saline or ethanol (2 g/kg, i.p.) once a day for seven days. DMS was dissected 16 hrs after the last injection. (A) Repeated ethanol administration did not alter the protein levels of NR1 or NR2A subunits in total DMS homogenates. Left, image is representative of n = 3 for NR1 (top) and n = 9 for NR2A (bottom). p > 0.05 vs. saline-treated rats (two-tailed t-test). (B) Repeated ethanol administration did not alter the protein levels of synaptosomal NR1 or NR2A subunits in the DMS. Left, image is representative of n = 6 (saline) and n = 6 (ethanol) for NR1 (top). n = 6 (saline) and n = 5 (ethanol) for NR2A (bottom). (A and B) Middle and right, bar graphs summarizing the averaged changes in protein levels of NR1 (middle) and NR2A subunits (right). Western blot data was normalized to GAPDH and plotted as percentage of saline treatment. p > 0.05 vs. saline-treated rats (two-tailed t-test). (C) Intermittent access to ethanol did not alter the protein levels of NR1 or NR2A in total DMS homogenates. Long Evans rats were exposed to 18 ethanol-drinking sessions under an intermittent access to 20% ethanol in a two-bottle-choice paradigm. One day after the last drinking session, DMS tissue was dissected out and protein levels of NR1 and NR2A subunits in homogenates were measured. Left, Image is representative of n = 7 (water). n = 8 (ethanol). Middle and right, bar graphs summarizing the averaged changes in protein levels of NR1 (middle) and NR2A subunits (right). Western blot data was normalized to GAPDH and plotted as percentage of water treatment. p > 0.05 vs. control (two-tailed t-test).