Table 4.
Effect of interfering substances on the performance of the HA ELISA
| Interfering substance & conc. tested | Specimen type | Conc. Unspiked (IU/ mL) | Conc. Spiked (IU/ mL) | Deviation observed (%) |
|---|---|---|---|---|
| Hemoglobin (486 mg/dL) | Negative | 8.8 | 12.0 | 36.4 |
| Low positive | 61.6 | 60.1 | 2.4 | |
| High positive | 719.6 | 760.0 | 5.6 | |
| Bilirubin (20.3 mg/dL) | Negative | 8.8 | 5.9 | 33.0 |
| Low positive | 61.6 | 48.5 | 21.3 | |
| High positive | 719.6 | 653.3 | 9.2 | |
| Lipemia* | ||||
| 2000 mg/dL | Negative | 8.8 | 7.9 | 10.2 |
| Low positive | 61.6 | 49.4 | 19.8 | |
| High positive | 719.6 | 703.2 | 2.3 | |
| 1000 mg/dL | Low positive | 61.6 | 62.3 | 1.1 |
| High positive | 719.6 | 710.1 | 1.3 | |
*
Evaluation of lipemia on the analytic performance of the HA ELISA was based on our in-house protocol as the exact conversion from 1460 Units suggested by the manufacturer to mg/dL could not be ascertained.