Skip to main content
. 2011 Nov 3;4(8):765–774.

Figure 3.

Figure 3

EPO promotes the proliferation, and inhibits the apoptosis of trophoblast cells and DSCs. Brdu proliferation assay was used to detect the proliferation of HTR-8/SVneo cells and primary human DSCs, which were treated with different concentrations of anti-human EPO neutralizing antibody (0, 0.2, 1 and 5 ng/ml) after 24, 48 or 72h. It was shown that the proliferation of HTR8/SVneo cells (A) and DSCs (B) is significantly lower as compared with that of the control (p<0.01). After incubated the HTR-8/SVneo cells and primary human DSCs with or without 1ug/ml anti-human EPO neutralizing antibody for 48h, the Annexin V and PI staining was used to detect the apoptosis of these cells. As shown in Figure, the apoptosis of HTR8/SVneo cells (C) and DSCs (D) is significantly higher as compared with that of the control (P<0.01). Results were highly reproducible in three independent experiments performed in triplicate. Error bars depict the standard error of the mean. *P<0.05, **P<0.01 compared to the control.