Fig. 3.

GW2580 reduces the proportion of macrophages in the CNS of mice with EAE. Brains and spinal cords from EAE mice treated prophylactically with vehicle or GW2580 were collected 17 days after MOG_35–55 immunization. Mononuclear cells were isolated and stained for T cells with anti-CD3 antibodies and for macrophages with anti-F4/80 antibodies. Results are representative of five mice per treatment group; for each treatment group, brain and spinal cords from 2–3 mice were pooled and analyzed by flow cytometry. Results are shown as the mean±SEM