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. Author manuscript; available in PMC: 2012 Nov 23.
Published in final edited form as: Chem Biol. 2011 Nov 23;18(11):1442–1452. doi: 10.1016/j.chembiol.2011.08.011

Table 1.

Aspulvinone analogs tested against five lucifease variants.

Cmp FLuc LmLuc Ultra RLuc VLuc
1 1.4±1.6 3.6±1.1 0.76±0.12 Inactive >57
2 3.6±1.5 2.5±0.5 >57 >57 inactive
3 1.8±0.9 1.9±0.8 19.0±4.6 >57 >57
4 0.72±0.40 0.50±0.20 4.1±1.8 Inactive 12.0±2.3
5 0.1±0.02 0.1±0.03 4.5±3.8 >57 6.5±3.3
8 1.7±1.6 5.3±5.9 33±2.1 Inactive 18±8
10 inactive inactive inactive >57 >57
11 inactive inactive inactive Inactive >57
12 18.6±8.8 14.8±2.8 31.7±3.0 Inactive inactive

All values are in μM; >57, %inhibition between 20–50% at the highest tested concentration of 57 μM; inactive, %inhibition <20% observed at the highest tested concentration. Data is mean ±SD values, from at least 4 determinations. FLuc = Photinus pyralis luciferase. Related luciferases: LmLuc = Luciola mingrelica luciferase (82% identical); Ultra = Ultra-Glo luciferase (derived from Photirus pennsylvanica (68% identical); unrelated luciferases: RLuc = Renilla reniformas luciferase; VLuc = Vibrio fischeri luciferase (Ye et al., 1997)