Table 1.
Cmp | FLuc | LmLuc | Ultra | RLuc | VLuc |
---|---|---|---|---|---|
1 | 1.4±1.6 | 3.6±1.1 | 0.76±0.12 | Inactive | >57 |
2 | 3.6±1.5 | 2.5±0.5 | >57 | >57 | inactive |
3 | 1.8±0.9 | 1.9±0.8 | 19.0±4.6 | >57 | >57 |
4 | 0.72±0.40 | 0.50±0.20 | 4.1±1.8 | Inactive | 12.0±2.3 |
5 | 0.1±0.02 | 0.1±0.03 | 4.5±3.8 | >57 | 6.5±3.3 |
8 | 1.7±1.6 | 5.3±5.9 | 33±2.1 | Inactive | 18±8 |
10 | inactive | inactive | inactive | >57 | >57 |
11 | inactive | inactive | inactive | Inactive | >57 |
12 | 18.6±8.8 | 14.8±2.8 | 31.7±3.0 | Inactive | inactive |
All values are in μM; >57, %inhibition between 20–50% at the highest tested concentration of 57 μM; inactive, %inhibition <20% observed at the highest tested concentration. Data is mean ±SD values, from at least 4 determinations. FLuc = Photinus pyralis luciferase. Related luciferases: LmLuc = Luciola mingrelica luciferase (82% identical); Ultra = Ultra-Glo luciferase (derived from Photirus pennsylvanica (68% identical); unrelated luciferases: RLuc = Renilla reniformas luciferase; VLuc = Vibrio fischeri luciferase (Ye et al., 1997)