Figure 2.

bFcRn overexpression resulted in a robust augmentation of the immune response in tg mice. FVB/N_tg4, FVB/N_tg5 (FVB/N transgenic mice carrying 4 and 5 copies of the bFcRn, respectively) and wild-type mice were immunized i.p. with OVA in CFA and challenged 14 days later with OVA in IFA. (A) OVA-specific IgM and (B) OVA-specific IgG titers were nearly tripled during the secondary immune response in FVB/N_tg4 and FVB/N_tg5 mice compared with the wild-type animals. (C) Transgenic mice produced significantly higher amounts of total IgG compared to the wild-type mice. (D) ELISPOT assays were performed to test for the presence of OVA-specific B cells. The number of OVA-specific cells was calculated taking account of the total spleen cell number. Multiple-fold increase of OVA-specific IgM and IgG producer cells was detected in the spleen of FVB/N_tg4 mice compared with wild-type controls. Significance levels indicate the difference between the tg and wild-type mice. Values shown are the mean ± SEM. (*p < 0.05; **p < 0.01; ***p < 0.001). All the experiments were repeated three times with similar results (Figure is reproduced from reference ²⁴ with permission. Copyright 2011. The American Association of Immunologists, Inc.).