Table 1.
Specific cytotoxic activities of mutants in CDRs
| WST (Raji, IC50 ± SD (ng/ml)) | Relative activity* | ||
| VHCDR3 | |||
| WT | 0.56 ± 0.16 | 1.00 | |
| H95A | 1.07 ± 0.05 | 0.52 | |
| S96A | 0.61 ± 0.12 | 0.92 | |
| G97A | >1000 | <0.0005 | |
| Y98A | >1000 | <0.0005 | |
| G99A | >1000 | <0.0005 | |
| T100A | ND | ND | |
| H100aA | 0.84 ± 0.04 | 0.67 | |
| W100bA | 83.6 ± 7.6 | 0.0067 | |
| G100cA | ND | ND | |
| V100dA | 1.00 ± 0.26 | 0.56 | |
| L100eA | 0.53 ± 0.12 | 1.06 | |
| F100fA | 1.03 ± 0.12 | 0.54 | |
| A101A | 0.56 ± 0.16 | 1.00 | |
| Y102A | 0.68 ± 0.09 | 0.82 | |
| VHCDR1 | |||
| WT | 0.61 ± 0.12 | 1.00 | |
| G26A | 0.91 ± 0.05 | 0.67 | |
| F27A | 1.01 ± 0.19 | 0.60 | |
| A28A | 0.61 ± 0.12 | 1.00 | |
| F29A | 0.69 ± 0.11 | 0.88 | |
| S30A | ND | ND | |
| I31A | 0.67 ± 0.03 | 0.91 | |
| Y32A | ND | ND | |
| D33A | ND | ND | |
| M34A | 1.34 ± 0.07 | 0.46 | |
| S35A | 0.63 ± 0.06 | 0.97 | |
| VLCDR1 | |||
| WT | 0.61 ± 0.09 | 1.00 | |
| R24A | 0.55 ± 0.08 | 1.11 | |
| A25A | 0.61 ± 0.09 | 1.00 | |
| S26A | 0.87 ± 0.18 | 0.70 | |
| Q27A | 1.17 ± 0.11 | 0.52 | |
| D28A | 1.64 ± 0.05 | 0.37 | |
| I29A | 0.66 ± 0.04 | 0.92 | |
| S30A | 1.38 ± 0.08 | 0.44 | |
| N31A | 0.86 ± 0.23 | 0.71 | |
| Y32A | 0.99 ± 0.07 | 0.62 | |
| L33A | 0.66 ± 0.16 | 0.92 | |
| N34A | 0.11 ± 0.07 | 5.55 | |
| VLCDR1 Position 34 | |||
| WT | 0.59 ± 0.06 | 1.00 | |
| N34G | 0.26 ± 0.13 | 2.23 | |
| N34A | 0.12 ± 0.10 | 4.92 | |
| N34Q | 0.40 ± 0.07 | 1.48 | |
| N34E | 11.02 ± 0.18 | 0.05 | |
| N34Y | 6.63 ± 0.12 | 0.09 | |
| N34H | 5.48 ± 0.05 | 0.11 | |
| N34S | 0.63 ± 0.16 | 0.94 |
*
Relative activity is calculated by IC50 of WT/IC50 of mutant. ND, not done.