Table 1.

Shigella spp. strains used in this study and number of MG1655 ORFs absent/divergent

Subgroup	Type No.	Strain	Abbreviation	Source*	No. of absent ORFs#
Dysenteriae	1	197	D1	CCDC	954
	2	G1252	D2	NKU	535
	3	G1281	D3	NKU	705
	4	G1190	D4	NKU	802
	5	G1213	D5	NKU	792
	6	G1192	D6	NKU	720
	7	G1222	D7	NKU	813
	8	G1221	D8	NKU	561
	9	G1274	D9	NKU	687
	10	G1292	D10	NKU	655
	11	G1246	D11	NKU	745
	12	G1263	D12	NKU	826
	13	G1271	D13	NKU	777
Flexneri	1a	571	F1a	CCDC	729
	1b	572	F1b	CCDC	694
	2a	301	F2a	CCDC	675
	2b	251	F2b	CCDC	685
	3	575	F3	CCDC	791
	4a	576	F4a	CCDC	668
	4b	577	F4b	CCDC	687
	5	246	F5	CCDC	678
	6	579	F6	CCDC	784
	Variant x	580	Fx	CCDC	735
	Variant y	581	Fy	CCDC	795
Boydii	1	G1228	B1	NKU	736
	2	G1184	B2	NKU	754
	3	G1232	B3	NKU	956
	4	227	B4	CCDC	774
	5	G1186	B5	NKU	476
	6	G1227	B6	NKU	765
	7	G1187	B7	NKU	549
	8	G1268	B8	NKU	835
	9	G1236	B9	NKU	528
	10	G1294	B10	NKU	850
	11	G1191	B11	NKU	588
	12	G1287	B12	NKU	794
	13	G1226	B13	NKU	896
	14	G1300	B14	NKU	907
	15	G1282	B15	NKU	595
	16	G1219	B16	NKU	589
	17	G1214	B17	NKU	663
	18	G1224	B18	NKU	881
Sonnei		46	SS	NICPBP	584

* NKU, NanKai University, Tianjin, China; CCDC, Chinese Center for Disease Control and prevention, Beijing, China; NICPBP, National Institute of the Control of Pharmaceutical and Biological Products, Beijing, China.

# Altogether, 2,245 ORFs were absent in at least one strain, and 137 ORFs were commonly absent in 43 strains.