Table 5.
Flux ranges for overall hydrogen ion (H+) exchanges and other related reactions.
| Network | Strain |
Overall H
+
exchange (mmol/h/gDW) |
Nitrite reductase flux (mmol/h/gDW) |
Urease flux (mmol/h/gDW) |
|||
|---|---|---|---|---|---|---|---|
| Min | Max | Min | Max | Min | Max | ||
| iNJ661v | Wild type | -1.99 | -1.93 | 1.00 | 1.00 | 0.00 | 0.00 |
| ΔNR | 0.04 | 0.07 | 0.00 | 0.00 | 0.00 | 0.00 | |
| iNJ661v | Wild type | -3.96 | -3.90 | 1.00 | 1.00 | 0.99 | 1.00 |
| with urea uptake |
ΔNR | -1.94 | -1.90 | 0.00 | 0.00 | 0.99 | 1.00 |
| iNJ661m | Wild type | 2.36 | 3.09 | 0.00 | 0.00 | 0.00 | 0.00 |
| ΔNR | 2.36 | 3.09 | 0.00 | 0.00 | 0.00 | 0.00 | |
| iNJ661m | Wild type | 0.43 | 0.91 | 0.00 | 0.00 | 0.99 | 1.00 |
| with urea uptake |
ΔNR | 0.43 | 0.91 | 0.00 | 0.00 | 0.99 | 1.00 |
Overall hydrogen ion (H+) exchange indicates the total H+ exchange between the environment and the Mycobacterium tuberculosis cells. A negative value of the exchange indicates that M. tuberculosis consumes H+ as a whole, while a positive value indicates that the metabolism generate a H+ surplus, i.e., increases acidification of the environment. The minimum (Min) and maximum (Max) fluxes reflected the ranges of the fluxes at the optimal growth and were obtained through flux variability analyses of iNJ661v and iNJ661 m. ΔNR represents the strain in which nitrite reductase (NR) was blocked. The unit of mmol/h/gDW represents mmol per hour per gram dry weight of M. tuberculosis.