. 2007 Jul 2;7:62. doi: 10.1186/1471-2180-7-62

Table 2.

Strains and plasmids

Strain	Relevant genotype	Phenotype	Origin, Reference
S. aureus
CHE482	CC45, ST45, SCCmec_N1(dfrA, fusB1), blaZ	Mc^r, Tm^r, Fa^r, Sx^R	IMM collection, University Zurich
ME21	CHE482ΔSCCmec_N1, blaZ	Mc^s, Tm^s, Fa^s, Sx^R	this study
CHE482Δ	CHE482Δ'SCCmec_N1(fusB1), blaZ	Mc^s, Tm^s, Fa^r, Sx^R	this study
ZH81	SCCmec_N1(dfrA, fusB1), blaZ	Mc^r, Tm^r, Fa^r	[9]
ME141	ZH81ΔSCCmec_N1, blaZ	Mc^s, Tm^s, Fa^s	this study
ZH4	SCCmec_N1(dfrA, fusB1), blaZ	Mc^r, Tm^r, Cp^r, Fa^r, Sx^R	[9]
ME135	ZH4ΔSCCmec_N1, blaZ	Mc^s, Tm^s, Cp^r, Fa^s, Sx^R	this study
ZH43	SCCmec_N1(dfrA, fusB1), blaZ, fusA	Mc^r, Tm^r, Cp^r, Fa^r	[9]
ME138	ZH43ΔSCCmec_N1, blaZ, fusA	Mc^s, Tm^s, Cp^rFa^r	this study
HDE288	Pediatric clone, type 4 ccr complex	Mc^r	[24, 43]
E. coli
DH5α	restriction-negative strain for cloning		Invitrogen
Plasmids
pYT3	ori(ts), S. aureus, tetL	Tc^r	[35]
pSR3-1	ori(ts) S. aureus, ccrAB2 genes, tetL	Tc^r	[35]
pAW17	S. aureus-E. coli shuttle vector, aac-aph	Km^r	[44]
pME15	pAW17 ccrAB4-1_CHE482	Km^r	This study
pME21	pYT3 ccrAB4-1_CHE482	Tc^r	This study
pME22	pYT3 ccrAB4-2_CHE482	Tc^r	This study

Abbreviations: Ap, ampicillin; CC, clonal complex; Cp, ciprofloxacin; Fa, fusidic acid; Gm, gentamicin; Km, kanamycin; Mc, methicillin; Ox, oxacillin; ST, sequence type; Sx, sulfomethoxazole; Tc, tetracycline; Tm, trimethoprim; ts, temperature sensitive.