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. 2011 Sep 5;9(9):1487–1501. doi: 10.3390/md9091487

Figure 3.

Figure 3

SDS-PAGE of recombinant Spi1C protein. The proteins were separated by 12% (w/v) SDS-PAGE, and then stained with Coomassie brilliant blue G-250. (A) Lane 1, molecular weight standards; lane 2, total protein of E. coli BL21(DE3)pLysS harboring empty pETBlue-2 as control; lane 3, total protein of E. coli BL21(DE3)pLysS harboring the recombinant spi1C in pETBlue-2 without induction by isopropyl-β-d-thiogalactopyranoside (IPTG); and lane 4, total protein of E. coli BL21(DE3)pLysS harboring the recombinant Spi1C in pETBlue-2 induced by the addition of 0.5 mM IPTG. The recombinant Spi1C protein is indicated by the black arrow; (B) Lane 1, molecular weight standards; lane 2, sample purified by the Ni-NTA column method.