Table 1. Molecular and Genetic results of the 30 new Adenoid Cystic Carcinomas.
Sample Number |
MYB-NFIB (new primer) |
3’RACEa | FISH | Q-PCR_MYB | |
---|---|---|---|---|---|
(exon 2-3) | (exon 15-16) | ||||
197-E8 |
MYB exon 9b- NFIB exon 11 MYB exon 9b- NFIB exon 12 |
N.P. | + | 10 | 1 |
327-B4 | MYB exon15- NFIB exon 12 | MYB exon 15- NFIB exon 12 | + | 62 | 0 |
236-A1 | MYB exon 15- NFIB exon 12 |
MYB exon 15- NFIB exon 12 MYB exon 15- NFIB exon 11 |
+ | 90 | 20 |
235-D4 |
MYB exon 9b- NFIB exon 11 MYB exon 9b- NFIB exon 12 |
N.P. | + | 161 | 5 |
290-F8 | - | MYB exon 15- NFIB 3’UTR | + | 162 | 1 |
381-C7 | MYB exon 8b- NFIB exon 11 | N.P. | + | 194 | 0 |
73-C3 |
MYB exon 15- NFIB exon 12 MYB exon 15- NFIB exon 11 |
N.P. | + | 266 | 18 |
394-D7 | - | MYB exon 15- NFIB 3’UTR | + | 358 | 0 |
404-D3 | MYB exon 8b- NFIB exon 12 | N.P. | + | 392 | 0 |
369-B5 | MYB exon 8b- NFIB exon 12 | N.P. | + | 760 | 4 |
133-C4 | MYB exon 8b- NFIB exon 12 | N.P. | + | 1516 | 33 |
163-D8 | - | N.P. | − | 0 | 0 |
537-G8 | - | N.P. | − | 0 | 1 |
124-C1 | - | N/A | + | 0 | 0 |
626-H6 | - | N.P. | − | 3 | 1 |
598-D6 | - | N.P. | − | 22 | 0 |
626-D1 | - | N.P. | − | 25 | 0 |
319-H5 | - | N.P. | − | 30 | 185 |
454-A8 | - | N.P. | − | 62 | 1 |
321-C6 | - | N.P. | − | 86 | 0 |
139-D5 | - | N.P. | − | 93 | 69 |
391-F7 | - | N/A | − | 133 | 388 |
383-H8 | - | N/A | − | 158 | 80 |
161-B4 | - | N/A | − | 226 | 150 |
610-H4 | - | N/A | + | 271 | 160 |
603-D6 | - | N.P. | − | 326 | 171 |
627-C2 | - | N/A | + | 342 | 180 |
392-B2 | - | N/A | + | 388 | 113 |
288-F7 | - |
MYB exon 13- MYB intron 13- EFR3A intron 22 (Chr8q24.22) |
+ | 416 | 6 |
78-C8 | - | N/A | + | 691 | 237 |
Molecular genetic results of the subset of ACC with alterations from previous study. | |||||
---|---|---|---|---|---|
Sample Number |
MYB-NFIB (new primer) |
3’RACEa | FISH | Q-PCR_MYB | |
(exon 2-3) | (exon 15-16) | ||||
405-B2 | - |
MYB exon 15- PDCDILG2 (chr 9; intron 3, inversion) |
+ | 106 | 0 |
b485-F7 | - | N/A | + | 231 | 421 |
233-C2 | - | N/A | + | 177 | 188 |
526-B5 | - | N/A | + | 368 | 213 |
471-F2 | - | N/A | + | 105 | 288 |
484-H3 | - | N/A | + | 105 | 213 |
594-D3 | - | N/A | + | 128 | 34 |
542-A1 | - | MYB exon 14- NFIB 3’UTR | + | 881 | 29 |
502-A5 | - | N/A | + | 606 | 384 |
185-G8 | MYB exon 11- NFIB exon 12 | MYB exon 11- NFIB exon 12 | + | 1264 | 635 |
335-C6 | MYB exon 8b- NFIB exon 12 | MYB exon 8b- NFIB exon 12 | + | 1229 | 5 |
191-D6 | MYB exon11- NFIB exon 12 | MYB exon 11- NFIB exon 12 | + | 488 | 62 |
318-H3 | - |
MYB exon/intron 11- NFIB 3’UTR |
+ | 0 | 0 |
391-D6 | MYB exon 12- NFIB exon 11 | MYB exon 15- NFIB exon 11 | + | 23 | 0 |
436-E2 |
MYB exon 8a- NFIB exon 12 MYB exon 8a- NFIB exon 11 |
MYB exon 8a- NFIB exon 12 | + | 1217 | 5 |
436-H3 | - | N/A | + | 229 | 476 |
570-H7 | MYB exon 8a- NFIB exon 12 | MYB exon 8a- NFIB exon 12 | + | 446 | 0 |
Note:
N.P., not performed; N/A, No-Abnormality (MYB gene is intact at 3’region). (−), negative; (+), positive.
Note:
N/A, No-Abnormality (MYB gene is intact at 3’region). (−), negative; (+), positive.
485-F7 sample had a NFIB-AIG1 gene fusion