Figure 4.

Mutations in the FMN riboswitch allows Listerial growth and expression of Imo1945 in the presence of roseoflavin. (A) Wild-type or M1 strains were grown for 10 hours in BHI in the absence or presence of roseoflavin at a concentration of 100 µM. Growth was measured by optical density (OD₆₀₀). Samples were compared to the control without added compound and shown as mean values with standard deviations (n = 6) and statistical analysis [Student t-test (two-tailed) (***p < 0.001)]. (B) L. monocytogenes wild-type or M1 strains were grown in BHI to an OD₆₀₀ = 0.25 when roseoflavin (Ro) or riboflavin (Ri) (100 µM) was added for ∼1.5 generations before RNA extraction. Northern blot was hybridized with PCR-generated, radioactively labeled DNA probes complementary to Rli96, hly and tmRNA (control), respectively.