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Treatment with roseoflavin results in a decreased expression of the virulence genes hly and actA in minimal medium. L. monocytogenes was grown in minimal medium to an OD600 = 0.25 when roseoflavin (Ro) or riboflavin (Ri) (100 µM) was added for ∼1.5 generations before RNA extraction. Northern blot was hybridized with PCR-generated, radioactively labeled DNA probes complementary to hly, actA and tmRNA (control), respectively.
